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Kystis is continuously working with customers to develop project-specific assays and the examples below have all been previously validated as moderate (96-well) throughput assays:
Functional differentiation is the key to the added value of the Biomimetic Urothelium over existing proliferative cell models. Several different strategies have been developed to look at urothelial differentiation within this tissue model.
Human urothelial tissues are naturally highly quiescent with very little cell division. This is one of the reasons proliferative cell cultures are poorly representative of the native tissue. The figure below shows the Alamar Blue assay used to illustrate the reduced cell division within the differentiated Biomimetic Urothelium.
Scratch wound healing assays have proved very informative. When conducted over short periods of time, induced proliferation can play no role and this allows easily quantifiable study of migration. The figure below shows how two compounds affect urothelial wound healing in opposite fashions depending on the differentiation state of the tissue. These results are from unpublished data and compounds will be revealed following publication.
Apoptosis can be monitored by any of the wide range of existing assays. We have characterised Alamar Blue, Live/Dead staining and an active caspase 3/7 assay for this purpose.