Supportive publications

Kystis' track record in research on the bladder and its epithelial lining, the urothelium, is exemplified by the research in these publications.



Baker SC, Shabir S, Southgate J. Biomimetic urothelial tissue models for the in vitro evaluation of barrier physiology and bladder drug efficacy. Mol Pharm. 2014; 11(7):1964-70.  PMID: 24697150. (review).

Böck M, Hinley J, Schmitt C, Wahlicht T, Kramer S, Southgate J. Identification of ELF3 as an early transcriptional regulator of human urothelium. Dev Biol. 2014; 386(2):321-30.
PMID: 24374157. 

Bullers S, Baker S, Ingham E, Southgate J. The human tissue:biomaterial interface: a role for PPARγ-dependent glucocorticoid receptor activation in regulating the CD163+ M2 macrophage phenotype. Tissue Eng Part A. 2014. (in press). PMID: 24548290. 

Garriboli M, Radford A, Southgate J.  Regenerative medicine in urology.  Eur J Pediatr Surg. 2014; 24(3): 227-36.  PMID: 24918400.

Georgopoulos NT, Kirkwood LA, Southgate J.  A novel bidirectional positive feedback loop between Wnt/β-catenin and EGFR/ERK: role of context-specific signalling crosstalk in modulating epithelial tissue regeneration. J Cell Science 2014; 127(pt 13): 2967-82.  PMID 24816560.  

Rebouissou S, Bernard-Pierrot I, de Reyniès A, Lepage M-L, Krucker C, Chapeaublanc E, Hérault A, Kamoun A, Caillaut A, Letouzé E, Elarouci N, Neuzillet Y, Denoux Y, Molinié V, Vordos D, Laplanche A, Maillé P, Soyeux P, Ofualuka K, Reyal F, Biton A, Sibony M, Paoletti X, Southgate J, Benhamou S, Lebret T, Allory Y, Radvanyi F. EGFR as a potential therapeutic target for a subset of muscle-invasive bladder cancers with a basal-like phenotype.  Science Translational Medicine 2014; 6(244).  PMID: 25009231.  (in press).

Wezel F, Pearson J, Southgate J. Plasticity of in vitro-generated urothelial cells for functional tissue formation. Tissue Eng Part A. 2014; 20(9-10): 1358-68. PMID: 24350594.othelialcell model. J R Soc In


Baker SC, Stahlschmidt J, Oxley J, Hinley J, Eardley I, Marsh F, Gillatt D, Fulford S, Southgate J. Nerve hyperplasia: a unique feature of ketamine. Acta Neuropathologica Comm 2013;1(64):1-7. PMID: 24252413.

Garthwaite M, Hinley J, Cross W, Warwick RM, Ambrose A, Hardaker H, Eardley I, Southgate J. Use of donor bladder tissues for in vitro research. BJU Int. 2013; 113(1): 160-6. PMID: 24053725.

Shabir S, Cross W, Kirkwood LA, Pearson JF, Appleby PA, Walker D, Eardley I, Southgate J. Functional expression of purinergic P2 receptors and transient receptor potential channels by the human urothelium. Am J Physiol Renal Physiol. 2013;305(3):F396-406. PMID: 23720349.

Wezel F, Pearson J, Kirkwood LA, Southgate J. Differential expression of Oct4 variants and pseudogenes in normal urothelium and urothelial cancer. Am J Pathol. 2013;183(4):1128-36. PMID: 23933063.

Wezel F, Southgate J. Reprogramming stromal cells from the urinary tract and prostate: a trip to pluripotency and back? Eur Urol. 2013;64(5):762-4. PMID: 23706568.

Wezel F, Pearson J, Southgate J.  Plasticity of in vitro-generated urothelial cells for functional tissue formation.  Tissue Engineering Part A. 2013; 20(9-10): 1358-68. 
PMID: 24350594.

Wezel F, Southgate J.  Tissue engineering using ceramics and polymers (2nd edition).  Tissue/organ regeneration:  the Bladder.  2013 (in press) (book chapter).


Fleming JM, Shabir S, Varley CL, Kirkwood LA, White A, Holder J, Trejdosiewicz LK, Southgate J. Differentiation-associated reprogramming of the transforming growth factor β receptor pathway establishes the circuitry for epithelial autocrine/paracrine repair. PLoS One. 2012;7(12):e51404. PMID: 23284691.

Ho JR, Chapeaublanc E, Kirkwood L, Nicolle R, Benhamou S, Lebret T, Allory Y, Southgate J, Radvanyi F, Goud B. Deregulation of Rab and Rab effector genes in bladder cancer. PLoS One. 2012;7(6):e39469. PMID: 22724020.

Rubenwolf PC, Georgopoulos NT, Kirkwood LA, Baker SC, Southgate J. Aquaporin expression contributes to human transurothelial permeability in vitro and is modulated by NaCl. PLoS One. 2012;7(9):e45339. PMID: 23028946.

Subramaniam R, Turner AM, Abbas SK, Thomas DF, Southgate J. Seromuscular grafts for bladder reconstruction: extra-luminal demucosalisation of the bowel. Urology. 2012;80(5):1147-50.
PMID: 23107403.

Walker DC, Southgate J. The modulatory effect of cell-cell contact on the tumorigenic potential of
pre-malignant epithelial cells: a computational exploration. J R Soc Interface. 2012. PMID:  23097504.


Aboushwareb T, McKenzie P, Wezel F, Southgate J, Badlani G. 2011. Is tissue engineering and biomaterials the future for lower urinary tract dysfunction (LUTD)/pelvic organ prolapse (POP)? Neurourol Urodyn, 30(5):775-82.

Baker SC, Southgate J.  2011. Bladder Tissue Regeneration.  Electrospinning for tissue regeneration, Bosworth & Downes (Eds): Woodhead Publishing Limited, 225-241 (book chapter).

Baker SC, Rohman G, Hinley J, Stahlschmidt J, Cameron NR, Southgate J. 2011.  Cellular integration and vascularisation promoted by a resorbable particulate-leached cross-linked poly(ε-caprolactone) scaffold.  Macromolecular Bioscience.  11(5), 618-27.

Georgopoulos NT, Kirkwood LA, Varley CL, Unwin RS, MacLaine N, Aziz N, Southgate J. 2011.  Immortalisation of normal human urothelial cells compromises differentiation.  Eur Urol. 60, 141-9.

Munkley J, Copeland NA, Moignard V, Knight JR, Greaves E, Ramsbottom SA, Pownall ME, Southgate J, Ainscough JF, Coverley D. 2011. Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells. Nucleic Acids Res. 39 (7), 2671-7.

Rubenwolf P, Southgate J. 2011.  Permeability of differentiated human urothelium in vitro.  Permeability Barrier – Methods and Protocols, Walker (Ed):  Springer. 763: 207.

Smith NJ, Varley CL, Eardley I, Feather S, Trejdosiewicz L, Southgate J.  2011. Toll-Like receptor responses of normal human urothelial cells to bacterial flagellin and lipopolysaccharide.  J Urol. 186, 1084-1092.

Subramaniam R, Hinley J, Stahlschmidt J, Southgate J. 2011. Tissue-Engineering Potential of Urothelial Cells from Abnormal Pediatric Bladders.  J Urol. 186, 2014-2020.

Turner A, Subramanian R, Thomas DFM, Hinley J, Abbas SK, Stahlschmidt J, Southgate J.  2010.  Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty.  Eur.Urol. 59, 447-454.

Varley CL, Southgate J.  2011. Organotypic and 3D reconstructed cultures of the human bladder and urinary tract. Methods Mol Biol. 695, 197-211.  

Vallot C, Stransky N, Bernard-Pierrot I, Herault A, Zucman-Rossi J, Chapeaublanc E, Vordos D, Laplanche A, Benhamou S, Lebret T, Southgate J, Allory Y, Radvanyi Francois. 2011. A novel epigenetic phenotype associated with the most aggressive pathway of bladder tumor progression. J Natl. Cancer Inst. 103, 47-60.

Wezel F, Southgate J, Thomas DFM.  2011.  Regenerative medicine in urology. Br J Urol Int. 108, 1046-1065.

Wood D, Cottrell A, Baker SC, Southgate J, Harris M, Fulford S, Woodhouse C, Gillatt D.  2011.  Recreational ketamine:  from pleasure to pain.  BJU.  107(12): 1881-4.

Understanding the Dynamics of Biological Systems:  Lessons Learned from Integrative Systems Biology, Dubitzky H, Southgate J, Fuβ H (Eds).  2011.  Springer. (edited book).


Georgopoulos NT, Kirkwood LA, Walker DC, Southgate J.  2010.   Differential regulation of growth-promoting signaling pathways by E-cadherin. PLoS ONE.  5, e13621.

Walker D, Georgopoulos NT, Southgate J. 2010. Anti-social cells: Predicting the influence of E-cadherin loss on the growth of epithelial cell populations. J Theor Biol 262, 425-440.


Baker SC, Rohman G, Southgate J, Cameron NR. 2009. The relationship between the mechanical properties and cell behaviour on PLGA and PCL scaffolds for bladder tissue engineering. Biomaterials 30, 1321-1328.

Chopra B, Georgopoulos NT, Nicholl A, Hinley J, Oleksiewicz MB, Southgate J. 2009. Structurally diverse peroxisome proliferator-activated receptor agonists induce apoptosis in human uro-epithelial cells by a receptor-independent mechanism involving store-operated calcium channels. Cell Prolif. 42, 688-700.

Egerod FL, Svendsen JE, Hinley J, Southgate J, Bartels A, Brunner N, Oleksiewicz MB. 2009. PPAR alpha and PPAR gamma coactivation rapidly induces Egr-1 in the nuclei of the dorsal and ventral urinary bladder and kidney pelvis urothelium of rats. Toxicol Pathol 37, 947-958.

Korossis S, Bolland F, Southgate J, Ingham E, Fisher J. 2009. Regional biomechanical and histological characterisation of the passive porcine urinary bladder: Implications for augmentation and tissue engineering strategies. Biomaterials 30, 266-275.

Rohman G, Baker SC, Southgate J, Cameron NR. 2009.  Heparin functionalisation of porous PLGA scaffolds for controlled, biologically relevant delivery of growth factors for soft tissue engineering.  J. Mater. Chem 19, 9265-9273.

Rubenwolf PC, Georgopoulos NT, Clements LA, Feather S, Holland P, Thomas DF, Southgate J. 2009. Expression and Localisation of Aquaporin Water Channels in Human Urothelium In Situ and In Vitro. Eur Urol. 56, 1013-1024.

Turner AM, Southgate J. (2009).  The Tissue-engineered Urinary Bladder:  A Regenerative and Developmental Challenge.  Fundamentals of Tissue Engineering and Regenerative Medicine, Meyer et al (Eds): Springer, 429-440. (book chapter).

Varley CL, Bacon EJ, Holder JC, Southgate J. 2009. FOXA1 and IRF-1 intermediary transcriptional regulators of PPARgamma-induced urothelial cytodifferentiation. Cell Death Differentiation 16, 103-114.

Walker DC, Southgate J. 2009. The virtual cell - a candidate co-ordinator for 'middle-out' modelling of biological systems. Brief Bioinform 10, 450-461. (review).


Baker SC, Southgate J. 2008. Towards control of smooth muscle cell differentiation in synthetic 3D scaffolds. Biomaterials 29, 3357-3366.

Bolland F, Southgate J. 2008. Bio-engineering urothelial cells for bladder tissue transplant. Expert Opin Biol Ther 8, 1039-1049. (review).

Chopra B, Hinley J, Oleksiewicz MB, Southgate J. 2008. Trans-species comparison of PPAR and RXR expression by rat and human urothelial tissues. Toxicol Pathol 36, 485-495.

MacLaine NJ, Wood MD, Holder JC, Rees RW, Southgate J. 2008. Sensitivity of normal, paramalignant, and malignant human urothelial cells to inhibitors of the epidermal growth factor receptor signaling pathway. Mol Cancer Res 6, 53-63.

Marsh F, Garthwaite MA, Southgate J, Ramage C. 2008. Assessing UK specialists' knowledge of the diagnostic criteria for painful bladder syndrome/interstitial cystitis. Int Urogynecol J Pelvic Floor Dysfunct 19, 615-620.

Oleksiewicz MB, Southgate J, Iversen L, Egerod FL. 2008. Rat Urinary Bladder Carcinogenesis by Dual-Acting PPARalpha + gamma Agonists. PPAR Res 2008, 103167.  (review).

Shabir S, Southgate J, 2008. Calcium signalling in wound-responsive normal human urothelial cell monolayers. Cell Calcium 44, 453-464.

Turner AM, Subramaniam R, Thomas DFM, Southgate J. 2008. Generation of a functional, differentiated porcine urothelial tissue in vitro. Eur Urol 54, 1423-1432.

Varley CL, Southgate J. 2008. Effects of PPAR agonists on proliferation and differentiation in human urothelium. Exp Toxicol Pathol 60, 435-441. (review).

Walker DC, Georgopoulos NT, Southgate J. 2008. From pathway to population - a multiscale model of juxtacrine EGFR-MAPK signalling. BMC Syst Biol 2, 102.

Wood D, Southgate J. 2008. Current status of tissue engineering in urology. Curr Opin Urol 18, 564-569. (review).


Bolland F, Korossis S, Wilshaw SP, Ingham E, Fisher J, Kearney JN, Southgate J. 2007. Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering. Biomaterials 28, 1061-1070.

Rohman G, Pettit JJ, Isaure F, Cameron NR, Southgate J. 2007. Influence of the physical properties of two-dimensional polyester substrates on the growth of normal human urothelial and urinary smooth muscle cells in vitro. Biomaterials 28, 2264-2274.

Southgate J, Varley CL, Garthwaite MA, Hinley J, Marsh F, Stahlschmidt J, Trejdosiewicz LK, Eardley I. 2007. Differentiation potential of urothelium from patients with benign bladder dysfunction. BJU Int 99, 1506-1516.

Turner AM, Subramanian R, Thomas DFM.  (2007).  Bladder Tissue Engineering. Tissue engineering using ceramics and polymers, Boccaccini & Gough (Eds):  Woodhead Publishing Limited, 445-465 (book chapter).


Baker SC, Atkin N, Gunning PA, Granville N, Wilson K, Wilson D, Southgate J. 2006. Characterisation of electrospun polystyrene scaffolds for three-dimensional in vitro biological studies. Biomaterials 27, 3136-3146.

Crallan RA, Georgopoulos NT, Southgate J. 2006. Experimental models of human bladder carcinogenesis. Carcinogenesis 27, 374-381.  (review).

Garthwaite MA, Thomas DFM, Subramaniam R, Stahlschmidt J, Eardley I, Southgate J. 2006. Urothelial differentiation in vesicoureteric reflux and other urological disorders of childhood: a comparative study. Eur Urol 49, 154-159, 159-160.

Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK. 2006. A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation. Cell Death Differ 13, 1789-1801.

Hall GD, Smith B, Weeks RJ, Selby PJ, Southgate J, Chester JD. 2006. Novel urothelium specific gene expression identified by differential display reverse transcriptase-polymerase chain reaction. J Urol 175, 337-342.

Jenkins D, Bitner-Glindzicz M, Malcolm S, Allison J, de Bruyn R, Flanagan S, Thomas DFM, Belk RA, Feather SA, Bingham C, Southgate J, Woolf AS. 2006. Mutation analyses of Uroplakin II in children with renal tract malformations. Nephrol Dial Transplant 21, 3415-3421.

Korossis S, Bolland F, Ingham E, Fisher J, Kearney J, Southgate J. 2006. Review: tissue engineering of the urinary bladder: considering structure-function relationships and the role of mechanotransduction. Tissue Eng 12, 635-644.  (review).

Steele LP, Georgopoulos NT, Southgate J, Selby PJ, Trejdosiewicz LK. 2006. Differential susceptibility to TRAIL of normal versus malignant human urothelial cells. Cell Death Differ 13, 1564-1576.

Stransky N, Vallot C, Reyal F, Bernard-Pierrot I, de Medina SG, Segraves R, de Rycke Y, Elvin P, Cassidy A, Spraggon C, Graham A, Southgate J, Asselain B, Allory Y, Abbou CC, Albertson DG, Thiery JP, Chopin DK, Pinkel D, Radvanyi F. 2006. Regional copy number-independent deregulation of transcription in cancer. Nat Genet 38, 1386-1396.

Varley CL, Garthwaite MA, Cross W, Hinley J, Trejdosiewicz LK, Southgate J. 2006. PPARgamma-regulated tight junction development during human urothelial cytodifferentiation. J Cell Physiol 208, 407-417.

Walker D, Wood S, Southgate J, Holcombe M, Smallwood R. 2006. An integrated agent-mathematical model of the effect of intercellular signalling via the epidermal growth factor receptor on cell proliferation. J Theor Biol 242, 774-789.


Cross WR, Eardley I, Leese HJ, Southgate J. 2005. A biomimetic tissue from cultured normal human urothelial cells: analysis of physiological function. Am J Physiol Renal Physiol 289, F459-468.

Hall GD, Weeks RJ, Olsburgh J, Southgate J, Knowles MA, Selby PJ, Chester JD. 2005. Transcriptional control of the human urothelial-specific gene, uroplakin Ia. Biochim Biophys Acta 1729, 126-134.

Hill GE, Fenwick S, Matthews BJ, Chivers RA, Southgate J. 2005. The effect of low-intensity pulsed ultrasound on repair of epithelial cell monolayers in vitro. Ultrasound Med Biol 31, 1701-1706.

Shaw NJ, Georgopoulos NT, Southgate J, Trejdosiewicz LK. 2005. Effects of loss of p53 and p16 function on life span and survival of human urothelial cells. Int J Cancer 116, 634-639.

Stahlschmidt J, Varley CL, Toogood G, Selby PJ, Southgate J. 2005. Urothelial differentiation in chronically urine-deprived bladders of patients with end-stage renal disease. Kidney Int 68, 1032-1040.

Varley C, Hill G, Pellegrin S, Shaw NJ, Selby PJ, Trejdosiewicz LK, Southgate J. 2005. Autocrine regulation of human urothelial cell proliferation and migration during regenerative responses in vitro. Exp Cell Res 306, 216-229.

Woodroffe PJ, King JR, Varley CL, Southgate J. 2005. Modelling cell signalling and differentiation in the urothelium. Bull Math Biol 67, 369-389.


Demotie A, Fairlamb IJ, Lu FJ, Shaw NJ, Spencer PA, Southgate J. 2004. Synthesis of jaspaquinol and effect on viability of normal and malignant bladder epithelial cell lines. Bioorg Med Chem Lett 14, 2883-2887.

Fraser M, Thomas DF, Pitt E, Harnden P, Trejdosiewicz LK, Southgate J. 2004. A surgical model of composite cystoplasty with cultured urothelial cells: a controlled study of gross outcome and urothelial phenotype. BJU Int 93, 609-616.

Kimuli M, Eardley I, Southgate J. 2004. In vitro assessment of decellularized porcine dermis as a matrix for urinary tract reconstruction. BJU Int 94, 859-866.

Varley CL, Stahlschmidt J, Lee WC, Holder J, Diggle C, Selb, PJ, Trejdosiewicz LK, Southgate J. 2004. Role of PPARgamma and EGFR signalling in the urothelial terminal differentiation programme. J Cell Sci 117, 2029-2036.

Varley CL, Stahlschmidt J, Smith B, Stower M, Southgate J. 2004. Activation of peroxisome proliferator-activated receptor-gamma reverses squamous metaplasia and induces transitional differentiation in normal human urothelial cells. Am J Pathol 164, 1789-1798.

Walker DC, Hill G, Wood SM, Smallwood RH, Southgate J. 2004. Agent-based computational modeling of wounded epithelial cell monolayers. IEEE Trans Nanobioscience 3, 153-163.

Walker DC, Southgate J, Hill G, Holcombe M, Hose DR, Wood SM, Mac Neil S, Smallwood RH. 2004. The epitheliome: agent-based modelling of the social behaviour of cells. Biosystems 76, 89-100.


Cross WR, Thomas DF, Southgate J. 2003. Tissue engineering and stem cell research in urology. BJU Int 92, 165-171.  (review).

Diggle CP, Cruickshank S, Olsburgh JD, Pellegrin S, Smith B, Banks RE, Selby PJ, Knowles MA, Southgate J, Harnden P. 2003. Identification of genes up-regulated in urothelial tumors: the 67-kd laminin receptor and tumor-associated trypsin inhibitor. Am J Pathol 163, 493-504.

Olsburgh J, Harnden P, Weeks R, Smith B, Joyce A, Hall G, Poulsom R, Selby P, Southgate J. 2003. Uroplakin gene expression in normal human tissues and locally advanced bladder cancer. J Pathol 199, 41-49.

Southgate J, Cross W, Eardley I, Thomas DF, Trejdosiewicz LK. 2003. Bladder reconstruction--from cells to materials. Proc Inst Mech Eng [H] 217, 311-316.  (review).


Booth C, Harnden P, Selby PJ, Southgate J. 2002. Towards defining roles and relationships for tenascin-C and TGFbeta-1 in the normal and neoplastic urinary bladder. J Pathol 198, 359-368.

Bugajska U, Georgopoulos NT, Southgate J, Johnson PW, Graber P, Gordon J, Selby PJ, Trejdosiewicz LK. 2002. The effects of malignant transformation on susceptibility of human urothelial cells to CD40-mediated apoptosis. J Natl Cancer Inst 94, 1381-1395.

Byrne AT, Southgate J, Brison DR, Leese HJ. 2002. Effects of insulin-like growth factors I and II on tumour-necrosis-factor-alpha-induced apoptosis in early murine embryos. Reprod Fertil Dev 14, 79-83.

Byrne AT, Southgate J, Brison DR, Leese HJ. 2002. Regulation of apoptosis in the bovine blastocyst by insulin and the insulin-like growth factor (IGF) superfamily. Mol Reprod Dev 62, 489-495.

Crallan RA, Lord PG, Rees RW, Southgate J., 2002. Inter-individual variation in urothelial DNA repair gene expression in vitro. Toxicol In Vitro 16, 383-387.

Olsburgh J, Weeks R, Selby P, Southgate J. 2002. Human uroplakin lb gene structure and promoter analysis. Biochim Biophys Acta 1576, 163-170.

Southgate J, Masters JR, Trejdosiewicz LK. Culture of Human Urothelium, In: Freshney RI and Freshney MG (Eds). Culture of Epithelial Cells. J Wiley and Sons, Inc. New York, 2002; pp 381-400 (book chapter).


Cruickshank SM, Southgate J, Trejdosiewicz LK. 2001. Normal human liver organ culture. In Vitro Cell Dev Biol Anim 37, 231-233.

Diggle C.P, Pitt E, Harnden P, Trejdosiewicz LK, Southgate J. 2001. Role of p53 in the responses of human urothelial cells to genotoxic damage. Int J Cancer 93, 199-203.

Scriven SD, Trejdosiewicz LK, Thomas DFM, Southgate J. 2001. Urothelial cell transplantation using biodegradable synthetic scaffolds. J Mater Sci Mater Med 12, 991-996.

Sillett HK, Cruickshank SM, Southgate J, Trejdosiewicz LK. 2001. Transforming growth factor-beta promotes 'death by neglect' in post-activated human T cells. Immunology 102, 310-316.

Smith BA, Kennedy WJ, Harnden P, Selby PJ, Trejdosiewicz LK, Southgate J. 2001. Identification of genes involved in human urothelial cell-matrix interactions: implications for the progression pathways of malignant urothelium. Cancer Res 61, 1678-1685.


Banks RE, Forbes MA, Patel PM, Storr M, Hallam S, Clarke D, Novick D, Ingham E, Bowmer C, Southgate J, Trejdosiewicz LK, Illingworth J, Perren TJ, Selby PJ. 2000. Subcutaneous administration of recombinant glycosylated interleukin 6 in patients with cancer: pharmacokinetics, pharmacodynamics and immunomodulatory effects. Cytokine 12, 388-396.

Diggle CP, Pitt E, Trejdosiewicz LK, Southgate J. 2000. Effects of long-chain fatty acids on human urothelial cells in organ culture. Prostaglandins Leukot Essent Fatty Acids 62, 249-252.

Diggle CP, Pitt E, Roberts P, Trejdosiewicz LK, Southgate J. 2000. N;-3 and n;-6 polyunsaturated fatty acids induce cytostasis in human urothelial cells independent of p53 gene function. J Lipid Res 41, 1509-1515.

Harnden P, Southgate J. 2000. Revised classification of urothelial neoplasms. Am J Surg Pathol 24, 160-162.

Sellar GC, Rabiasz GJ, Smith BA, Southgate J. Differential Display. In: Bartlett J (Ed). Methods in Molecular Medicine: Ovarian Cancer. Humana Press, 2000; pp 459-468. (book chapter).


Booth C, Harnden P, Selby PJ, Trejdosiewicz LK, Southgate J. 1999. The role of matrix metalloproteinases in an in vitro model of bladder tumor invasion. Adv Exp Med Biol 462, 413-417.

Byrne AT, Southgate J, Brison DR, Leese HJ. 1999. Analysis of apoptosis in the preimplantation bovine embryo using TUNEL. J Reprod Fertil 117, 97-105.

Comer MT, Shires M, Goode NP, Leese HJ, Trejdosiewicz LK, Southgate J. 1999. Expression of an antigen associated with basal bodies of human ciliated epithelial cells. Histochem J 31, 39-43.

Comer MT, Andrew AC, Leese HJ, Trejdosiewicz LK, Southgate J. 1999. Application of a marker of ciliated epithelial cells to gynaecological pathology. J Clin Pathol 52, 355-357.

De Medina SG, Popov Z, Chopin DK, Southgate J, Tucker GC, Delouvee A, Thiery JP, Radvanyi F. 1999. Relationship between E-cadherin and fibroblast growth factor receptor 2b expression in bladder carcinomas. Oncogene 18, 5722-5726.

Harnden P, Mahmood N, Southgate J. 1999. Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet 353, 974-977.

Harnden P, Kennedy W, Andrew AC, Southgate J. 1999. Immunophenotype of transitional metaplasia of the uterine cervix. Int J Gynecol Pathol 18, 125-129.

Protheroe AS, Banks RE, Mzimba M, Porter WH, Southgate J, Singh PN, Bosomworth M, Harnden P, Smith PH, Whelan P, Selby PJ. 1999. Urinary concentrations of the soluble adhesion molecule E-cadherin and total protein in patients with bladder cancer. Br J Cancer 80, 273-278.

Sillett HK, Southgate J, Howdle PD, Trejdosiewicz LK. 1999. Expression of activation and costimulatory elements by human intestinal intraepithelial lymphocytes. Scand J Immunol 50, 52-60.

Southgate J, Harnden P, Trejdosiewicz LK. 1999. Cytokeratin expression patterns in normal and malignant urothelium: a review of the biological and diagnostic implications. Histol Histopathol 14, 657-664. (review).

Southgate J, Harnden P, Selby PJ, Thomas DF, Trejdosiewicz LK. 1999. Urothelial tissue regulation. Unraveling the role of the stroma. Adv Exp Med Biol 462, 19-30.  (review).

Southgate J, Harnden P. 1999. Re: Cytokeratin 20: a new marker for early detection of bladder cell carcinoma. J Urol 162, 501-502.


Comer MT, Leese HJ, Southgate J. 1998. Induction of a differentiated ciliated cell phenotype in primary cultures of Fallopian tube epithelium. Hum Reprod 13, 3114-3120.

Cruickshank SM, Southgate J, Selby PJ, Trejdosiewicz LK. 1998. Expression and cytokine regulation of immune recognition elements by normal human biliary epithelial and established liver cell lines in vitro. J Hepatol 29, 550-558.

Lobban ED, Smith BA, Hall GD, Harnden P, Roberts P, Selby PJ, Trejdosiewicz LK, Southgate J. 1998. Uroplakin gene expression by normal and neoplastic human urothelium. Am J Pathol 153, 1957-1967.

Metcalf KS, Selby PJ, Trejdosiewicz LK, Southgate J. 1998. Culture of ascitic ovarian cancer cells as a clinically-relevant ex vivo model for the assessment of biological therapies. Eur J Gynaecol Oncol 19, 113-119.

Southgate J, Lobban D, Harnden P. 1998. Expression of cytokeratin 20 in urinary cytology of patients with bladder carcinoma. Cancer 83, 1052-1054.

Trejdosiewicz LK, Morton R, Yang Y, Banks RE, Selby PJ, Southgate J. 1998. Interleukins 4 and 13 upregulate expression of cd44 in human colonic epithelial cell lines. Cytokine 10, 756-765.


Booth C, Harnden P, Trejdosiewicz LK, Scriven S, Selby PJ, Southgate J., 1997. Stromal and vascular invasion in an human in vitro bladder cancer model. Lab Invest 76, 843-857.

Harnden P, Southgate J. 1997. Cytokeratin 14 as a marker of squamous differentiation in transitional cell carcinomas. J Clin Pathol 50, 1032-1033.

Metcalf KS, SP, Trejdosiewicz LK, Southgate J. 1997. Interferon induced changes in human ovarian carcinoma cell lines. Int J Gynaecol Cancer 7, 355-363.

Scriven SD, Booth C, Thomas DFM, Trejdosiewicz LK, Southgate J. 1997. Reconstitution of human urothelium from monolayer cultures. J Urol 158, 1147-1152.

Southgate J, Trejdosiewicz LK. Immunolabelling of cells and tissues: approaches and pitfalls. Hum Reprod 1997; 12s: 65-75. (review).


Dickens CJ, Comer MT, Southgate J, Leese HJ. 1996. Human Fallopian tubal epithelial cells in vitro: establishment of polarity and potential role of intracellular calcium and extracellular ATP in fluid secretion. Hum Reprod 11, 212-217.

Harnden P, Southgate J. 1996. Oestrogen receptor status of mast cells in interstitial cystitis. Br J Urol 77, 474-477.

Harnden P, Eardley I, Joyce AD, Southgate J. 1996. Cytokeratin 20 as an objective marker of urothelial dysplasia. Br J Urol 78, 870-875.

Pittman K, Burchill S, Smith B, Southgate J, Joffe J, Gore M, Selby P. 1996. Reverse transcriptase-polymerase chain reaction for expression of tyrosinase to identify malignant melanoma cells in peripheral blood. Ann Oncol 7, 297-301.

Southgate J, Pitt E, Trejdosiewicz LK. 1996. The effects of dietary fatty acids on the proliferation of normal human urothelial cells in vitro. Br J Cancer 74, 728-734.


Burchill SA, Bradbury MF, Pittman K, Southgate J, Smith B, Selby P. 1995. Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction. Br J Cancer 71, 278-281.

Harnden P, Allam A, Joyce AD, Patel A, Selby P, Southgate J. 1995. Cytokeratin 20 expression by non-invasive transitional cell carcinomas: potential for distinguishing recurrent from non-recurrent disease. Histopathology 27, 169-174.

Southgate J, Trejdosiewicz LK, Smith B, Selby PJ. 1995. Patterns of splice variant CD44 expression by normal human urothelium in situ and in vitro and by bladder-carcinoma cell lines. Int J Cancer 62, 449-456.

Southgate J, Proffitt J, Roberts P, Smith B, Selby P. 1995. Loss of cyclin-dependent kinase inhibitor genes and chromosome 9 karyotypic abnormalities in human bladder cancer cell lines. Br J Cancer 72, 1214-1218.

Southgate J, Kennedy W, Hutton KA, Trejdosiewicz LK. 1995. Expression and in vitro regulation of integrins by normal human urothelial cells. Cell Adhes Commun 3, 231-242.

Stanley AJ, Banks RE, Southgate J, Selby PJ. 1995. Effect of cell density on the expression of adhesion molecules and modulation by cytokines. Cytometry 21, 338-343.


Southgate J, Hutton KA, Thomas DF, Trejdosiewicz LK.1994. Normal human urothelial cells in vitro: proliferation and induction of stratification. Lab Invest 71, 583-594.


Dickens CJ, Southgate J, Leese HJ. 1993. Use of primary cultures of rabbit oviduct epithelial cells to study the ionic basis of tubal fluid formation. J Reprod Fertil 98, 603-610.

Hutton KA, Trejdosiewicz LK, Thomas DFM, Southgate J. 1993. Urothelial tissue culture for bladder reconstruction: an experimental study. J Urol 150, 721-725.


Iggo RD, Jamieson DJ, MacNeill SA, Southgate J, McPheat J, Lane DP. 1991. p68 RNA helicase: identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts. Mol Cell Biol 11, 1326-1333.

Smith B, Selby P, Southgate J, Pittman K, Bradley C, Blair GE. 1991. Detection of melanoma cells in peripheral blood by means of reverse transcriptase and polymerase chain reaction. Lancet 338, 1227-1229.


Iggo R, Picksley S, Southgate J, McPheat J, Lane DP. 1990. Identification of a putative RNA helicase in E.coli. Nucleic Acids Res 18, 5413-5417.


Thompson TC, Southgate J, Kitchener G, Land H. 1989. Multistage carcinogenesis induced by ras and myc oncogenes in a reconstituted organ. Cell 56, 917-930.


Hodges GM, Southgate J, Toulson EC. 1987. Colloidal gold--a powerful tool in scanning electron microscope immunocytochemistry: an overview of bioapplications. Scanning Microsc 1, 301-318.

Southgate J, Williams HK, Trejdosiewicz LK, Hodges GM. 1987. Primary culture of human oral epithelial cells. Growth requirements and expression of differentiated characteristics. Lab Invest 56, 211-223.

Trejdosiewicz LK, Malizia G, Badr-el-Din S, Smart CJ, Oakes DJ, Southgate J, Howdle PD, Janossy G, Poulter LW, Losowsky MS. 1987. T cell and mononuclear phagocyte populations of the human small and large intestine. Adv Exp Med Biol 216A, 465-473.

Wiedmann KH, Trejdosiewicz LK, Southgate J, Thomas HC. 1987. Human hepatocellular carcinoma: cross-reactive and idiotypic antigens associated with malignant transformation of epithelial cells. Hepatology 7, 543-550.


Trejdosiewicz LK, Southgate J, Kemshead JT, Hodges GM. 1986. Phenotypic analysis of cultured melanoma cells. Expression of cytokeratin-type intermediate filaments by the M5 human melanoma cell line. Exp Cell Res 164, 388-398.


Hodges GM, Carr KE, Hume SP, Marigold JC, Southgate J, Marshall JF. 1985. Changes in surface structure and concanavalin A-binding capacity of urothelium in the mouse bladder after whole-body neutron irradiation. Scan Electron Microsc, 1603-1614.

Nicholls RJ, Southgate J, Trejdosiewicz LK. 1985. Antibodies to normal human colon membranes: preparation, characterization and tissue distribution. Histochem J 17, 717-729.

Trejdosiewicz LK, Southgate J, Hodges GM, Goodman SL. 1985. Micro-heterogenous expression of peanut agglutinin-binding sites in the extracellular matrix of cultured cells. Exp Cell Res 156, 153-163.

Trejdosiewicz LK, Southgate J, Donald JA, Masters JR, Hepburn PJ, Hodges GM. 1985. Monoclonal antibodies to human urothelial cell lines and hybrids: production and characterization. J Urol 133, 533-538.

Wigley CB, Trejdosiewicz LK, Southgate J, Coventry R, Ozanne B. 1985. Growth factor production during multistage transformation of epithelium in vitro. I. Partial purification and characterisation of the factor(s) from a fully transformed epithelial cell line. J Cell Physiol 125, 156-165.